Jinhua Qinggan Granule UHPLC-Q-extractive-Orbitrap-MS assay: Putative identification of 45 potential anti-Covid-19 constituents, confidential addition, and pharmacopoeia quality-markers recommendation

The study combined UHPLC-Q-Orbitrap-MS analysis with authentic standards, to create a novel strategy for isomers recognition and putative identification. Through the strategy, anti-Covid-19 Jinhua Qinggan Granule was found comprise 28 45 potential constituents. detection of three constituents (Danshensu, cryptotanshin, tanshinone IIA) suggests Danshen as confidential additive. Based on this, 6 are recommended quality-marker candidates, including chlorogenic acid, acteoside, peimisine, baicalein, licoricesaponin H2, IIA. Obviously, can not only help public really understand Granule's formula chemistry, but also facilitate its Pharmacopoeia collection in future.